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. 2023 Feb 4;14:616. doi: 10.1038/s41467-023-36168-x

Fig. 6. Interfering with La influences bone resorption by human osteoclasts.

Fig. 6

a Illustration depicting the use of fluoresceinamine-labeled chondroitin sulfate trapped in calcium phosphate coated plates to assay bone resorption. b Bone resorption in osteoclasts overexpressing uncleavable La (D371A, D374A) or truncated La (1-375) normalized to that for the osteoclasts transfected with empty plasmid. (n = 3) (P = 0.06 and 0.05). c Bone resorption in osteoclasts transfected with siRNA targeting La transcript normalized to that for non-targeted siRNA. (n = 5) (P = 0.005). d Bone resorption in osteoclasts exposed to different concentrations of α-La mAb normalized to that for the cells treated with isotype control IgG at 7.5 µg/ml. (n = 3) (P = 0.0723, 0.0098 and 0.004). e Bone resorption in osteoclasts treated with 40 nM recombinant La 1-375 normalized to the control after adding the same amount of PBS. (n = 4) (P = 0.03) b–e Statistical significance was assessed via one-tailed paired t-test. Data are presented as mean values + /- SEM. Source data are provided as a Source Data file.