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. 2023 Feb 4;14:616. doi: 10.1038/s41467-023-36168-x

Fig. 7. Osteoclast formation in human osteoblast/osteoclast precursor co-culture depends on La protein.

Fig. 7

a Schematic of the multi-well configuration of the human osteoblast/osteoclast co-culture system used. Multi-well dividers were removed following the M-CSF derivation of human monocytes, and osteoclast precursors and primary osteoblast media intermixed. (Grey=Phalloidin-Alexa488, Cyan=Hoechst). b Representative immunofluorescence images comparing osteoclast fusion in the presence of 6 μg/ml control IgG or α-La mAb antibodies. Arrowheads denote syncytia with ≥3 nuclei. c Quantification of osteoclast fusion in the co-cultures in the presence of α-La mAb normalized to that in the presence of control IgG. (n = 4) (P = 0.03) Statistical significance was assessed via one-tailed paired t-test. Data are presented as mean values + /- SEM. Source data are provided as a Source Data file.