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. 2023 Feb 4;9:43. doi: 10.1038/s41420-022-01294-w

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© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A, B Western blot detected the protein level of apoptosis gene Bcl-2 and Bax, as well as NUSN2 (n = 3). C TUNEL detected the apoptosis of H9C2 cells after DOX treatment (n = 3, Bar = 20 µm). D Western blot detected the transfection efficiency of siNSUN2 in H9C2 cells (n = 3); E Western blot detected the effect of interfering of NSUN2 on apoptosis-related proteins in H9C2 cells after DOX treatment (n = 3). F TUNEL detected the effect of NSUN2 interference on DOX-induced apoptosis (n = 3, Bar = 20 µm). G Western blot detected the efficiency of NSUN2 overexpression in H9C2 cells. H TUNEL detected the effect of NSUN2 overexpression on DOX-induced H9C2 cell apoptosis (n = 3, Bar = 20 µm). I, J Effects of interference and overexpression of NSUN2 on the proliferation of H9C2 cells. The measurement data were shown as mean ± standard deviation and compared by Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001.