Figure 1.

Experimental approach. (a) Day old PC (n = 12) and WL (n = 12) chicks were experimentally infected with v2001 by intraabdominal inoculation and housed with naïve contact chicks (n = 8/chicken line) considered “experimental infection”. (b) After 2 weeks, MDV is shed into the environment, and naïve contact birds are infected considered “natural infection”. (c) Both “Infected”, based on fluorescent feathers and “Diseased”, based on clinical disease, were used for collection of whole lung lavage cell (WLLC) and liver sample collection. (d) RNA was extracted, cDNA synthesized, and RT-qPCR was performed to determine differential mRNA expression of PRs.