Table 1.
Purinergic receptors primer sets used for RT-qPCR assays.
| Family | Gene Name | Gene ID1 | Accession No.2 | Primer3 | Sequence (5’-3’) | Fragment (bp)4 | Source5 |
|---|---|---|---|---|---|---|---|
| P1 | ADORA1 | 374212 | NM_204316.4 | F.433 | ATCAATATCGGACCGCAGAC | 159 | This report |
| R.591 | GGGTGTCACCACGCTCTTAT | ||||||
| ADORA2A | 427705 | XR_005854444.1 | F.1574 | CATTGTTGGGTTGTTTGCAG | 243 | This report | |
| R.1816 | GTTCCTGCTTTGAACTGCTC | ||||||
| ADORA2B | 395971 | NM_205087.2 | F.969 | GGACTTCCGCTACACCTTCC | 106 | This report | |
| R1074 | GTTGGTGACAGTCAGGTGCT | ||||||
| ADORA3 | 373956 | NM_204151.3 | F.375 | GCATCCCGTTCCATTTCTGC | 315 | This report | |
| R.689 | TGAGAGTCCACGCGAAGAAC | ||||||
| P2X | P2X1 | 395190 | NM_204519.2 | F.312 | ATACATCATAGGGTGGGTTT | 359 | 39 |
| R.670 | AGATTTCGCAGGTCTTCAT | ||||||
| P2X2 | 101749217 | XM_004934420.2 | F.512 | CGCAGTTCACCATCCTCATC | 338 | 39 | |
| R.849 | CTTGGCAAACCTGTAGTTGTAGC | ||||||
| P2X3 | 428856 | NM_001397208.2 | F.433 | CTCGGTGTTCGTGGTGGTC | 377 | This report | |
| R.809 | GCGGCTGTTGCTCGGGAT | ||||||
| P2X4 | 374166 | NM_204291.2 | F.529 | CTATCACATACCCAATCCG | 413 | 39 | |
| R.941 | TTTCAATGCCACTACTATCC | ||||||
| P2X5 | 395507 | NM_204748.2 | F.714 | TCCTACCTAAAGACCTGCCACT | 345 | 39 | |
| R.1058 | TGCCTTGCCATTCACCAT | ||||||
| P2X6 | 429367 | XM_040685095.1 | F.993 | CAGCCACGCTACTCCTTCAT | 207 | This report | |
| R.1199 | GGTTCCAAGGAATGCGATGC | ||||||
| P2X7 | 771952 | XM_001235162.6 | F.1300 | TCCTATGATCCTCGCACCT | 378 | 39 | |
| R.1677 | GATGATGGCTCTGTCCTCC | ||||||
| P2Y | P2Y1 | 396275 | NM_205333.2 | F.326 | ACCTGCCCACCGTCTACATC | 348 | 39 |
| R.673 | CGGCGTTCTTCTTCTTCAGC | ||||||
| P2Y2 | 428108 | XM_025146950.2 | F.38 | TTTCTGCCTGCTTTACCGTCA | 490 | This report | |
| R.527 | AACATGTACGTGGTGGAGGC | ||||||
| P2Y3/P2Y6 | 396114 | NM_205195.1 | F.484 | AGTGCCTGCCCACCTTTGT | 240 | 39 | |
| R.723 | CGGCCTTGTCCTTCTTCTTG | ||||||
| P2Y4 | 100857687 | XM_004940541.4 | F.13 | CCGTCATTAAGCTGTCCGCA | 370 | This report | |
| R.382 | CTCCTCGTTGAAGACGCACT | ||||||
| P2Y5 | 396118 | NM_205199.3 | F.556 | AACAACACGGAGCAAAGAA | 351 | 39 | |
| R.906 | CAGGGTGACAGGGTACATAG | ||||||
| P2Y8 | 418665 | NM_001008679.2 | F.669 | TGGAAAGCACGGATCTGACC | 569 | This report | |
| F.1227 | AGGCCATCCATAGGTCCACT | ||||||
| P2Y10 | 422148 | XM_040669411.1 | F.218 | GCAACTGGACTTGCTCTGAT | 653 | This report | |
| R.870 | GCTGTTGTTTTGCAGCGGTA | ||||||
| P2Y13 | 107049027 | XM_040679499.1 | F.493 | GCATAGTGCTCCTCGGTCTC | 662 | This report | |
| R.1154 | TGTGTCCAGTTCGGTTCTCG | ||||||
| P2Y14 | 101749627 | NM_001317401.2 | F.481 | ACTCCAGCACAAACTCCTCG | 524 | This report | |
| R.1004 | CTCGCTTTTAAGGCCGATGC |
1National Center for Biotechnology Information (NCBI) Gene ID for Gallus gallus (chicken).
2NCBI mRNA Accession No.
3Primer direction (F-forward; R-reverse) and hybridization position in gene.
4Amplicon size in base pairs.
5Source of primer sequence.