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. 2023 Feb 4;14:610. doi: 10.1038/s41467-023-35995-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The t-SNE plot showing the unsupervised clustering results with cell types annotated. b The t-SNE plot showing the sample locations of single cells from P14. c The t-SNE plot showing the sample locations of single cells from P15. d, e Pseudotime trajectory analysis of tumour cells from P14 (d) and P15 (e). The left panel shows the t-SNE plot of tumour cells coloured according to the sample locations, the middle panel shows the evolutionary trajectory of tumour cells, and the right panel shows the corresponding phylogenetic tree. Source data are provided as a Source Data file. Pri, LLN, RLN, ILN, LALN, and RALN: primary tumour, left regional lymph nodes, right regional lymph nodes, inguinal lymph nodes, left axillary lymph nodes and right axillary lymph nodes, respectively.