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. 2023 Feb 4;14:608. doi: 10.1038/s41467-023-36324-3

Fig. 1. Systemic and local administration of GECIs using PHP.eB AAVs.

Fig. 1

A Experimental overview indicating the two injection methods, and the approaches used to monitor the expression. Illustration created with Biorender.com. B Post-mortem histological verification of jGCaMP8s expression 10 weeks after RO injection and microglia activation verified by Iba1-positive labeling. Scale bar indicates 200 µm. C Example images from in vivo two-photon microscopy of jGCaMP8s expressed by RO or IC virus injections 2, 4, 6, and 10 weeks after injection. Scale bar indicates 200 µm. D GECI expression at different depths in cortex after RO injection of jGCaMP8s. Scale bar indicates 150 µm.