Table 1.
Overview of initial GECI screening with virus titer and Addgene reference indicated
| GECI | Titer (VG/ml) | Detectable at 50 mW (RO injection) | Addgene plasmid # |
|---|---|---|---|
| GCaMP6f | 2.44E + 13 | No | 100837 |
| EE-RR- GCaMP6f | 2.04E + 13 | No | 158756 |
| jGCaMP7f | 2.04E + 13 | No | 104488 |
| EE-RR- GCaMP7f | 1.53E + 13 | No | 158760 |
| jGCaMP7s | 1.11E + 13 | Yes | 104487 |
| jGCaMP8f | 1.04E + 13 | No | 162376 |
| jGCaMP8m | 9.43E + 12 | Yes | 162375 |
| jGCaMP8s | 1.49E + 13 | Yes | 162374 |
| jREX-GECO1 | 9.36E + 12 | Yes | 169259a |
| Ribo-GCaMP6m | 1.86E + 13 | No | 158777 |
| CAG-mNeonGreen | 1.09E + 13 | Yes | 99134 |
ajREX-GECO1 expressed from a hSyn promoter was made and used in this manuscript.
All constructs were tested and confirmed viable for imaging using IC injections (with the exception of Ribo-GCaMP6m, where we did not observe in vivo expression). “Detectable at 50 mW (RO injections)” is defined as whether a single imaging plane using a 16× Nikon objective and 50 mW laser power at 920 nm gives clear single-cell fluorescence transients 6 weeks after injection. The fluorescent protein mNeongreen expressed under a CAG promoter was used as a positive expression control for the RO injection method.