Figure 3. ASXL1 Deletion Does Not Substantially Impact the Landscape of Covalent Histone Modifications in Developing Myeloid Cells.

A. Bone marrow from Asxl1^WT and Asxl1^Δ/Δ mice was harvested 6 months after induction of recombination via poly I:C administration, maturing granulocyte lineage cells isolated by FACS, and subjected to CUT&Tag for H3K27me3. Spearman Correlation of Genome-wide H3K27me3 signal in indicated bone marrow sub-populations. B. Representative tracks of H3K27me3 signal in indicated bone marrow sub-populations. C. Mean normalized read density at H3K27me3 peaks in indicated bone marrow sub populations. D. RPKM values for reads in peaks for indicated histone marks in CD133 purified granulocytic progenitors. E. Mean normalized read density for indicated histone marks at Myc target genes as measured by CUT&Tag in CD133-granulocytic progenitors from CausalPath analysis in Figure 2.