Figure 4. ASXL1 Deletion Leads to an Increase in RNAPII Pause-Release.

A. Spearman correlation of histone mark or RNAPII CUT&Tag in CD133 positive granulocyte progenitors from Asxl1^WT mice (n=2–4/group) and published ASXL1 ChIP-seq. B. Schematic depicting the calculation of RNAPII pause-index. C. Cumulative distribution of RNAPII pause-indices at genes bound by Myc and RNAPII in Asxl1^WT and Asxl1^Δ/Δ mice (n=4/group). D. Cumulative distribution of RNAPII pause-indices at genes bound by RNAPII and lacking a Myc peak in Asxl1^WT and Asxl1^Δ/Δ mice (n=4/group). Median pause index difference between Asxl1^WT and Asxl1^Δ/Δ conditions. Statistical significance between RNAPII pause-index distributions in Asxl1^WT and Asxl1^Δ/Δ conditions evaluated using a KS-test. E. Mean signal at all genes bound by RNAPII. Representative RNAPII tracks from genes with differential RNAPII pausing in from Asxl1^WT and Asxl1^Δ/Δ mice.