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. 2000 Jun;64(2):239–280. doi: 10.1128/mmbr.64.2.239-280.2000

FIG. 17.

FIG. 17

Translational control by HCV. (A) Structural representation of the HCV polyprotein. The individual positions of the polyprotein cleavage products are shown. NS5A (black region) from IFN-resistant HCV can bind and repress PKR (129, 133). (B) NS5A blocks PKR-dependent eIF2α phosphorylation. eIF2α phosphorylation from control (Neo) NIH 3T3 cell lines and those stably expressing NS5A from IFN-resistant HCV (NS5A-1A) or a nonfunctional NS5A mutant (ΔISDR) was assessed by single-dimension isoelectric focusing of cell extracts and anti-eIF2α immunoblot analysis (133). Cells were mock infected (lanes 1, 3, and 5) or infected with VSV (lanes 2, 4, and 6). Arrows denote the positions hypo- and hyper-phosphorylated isoforms of eIF2α (eIF2α and eIF2αP, respectively). Hyperphosphorylated eIF2α is phosphorylated on serine 51 by PKR and is sufficient to block mRNA translation (61, 89).