Table 2. The CO1 and 12S primers used and designed for the target species of interest.

Target species	Gene	Primer name	Tm (°C)	Sequence 5′–3′	Reference
Metazoan diversity	CO1	F: mlCOIintF, R: jgHCO2198	51.2 46.4	GGWACWGGWTGAACWGTWTAYCCYCC TAIACYTCIGGRTGICCRAARAAYCA	Leray et al. (2013)
Red hind	CO1	Epine_gutt_For, Epine_gutt_Rev	57.6 57.4	ACCCGCATGGGCTAGATTTC TTCGTCTGGAGTTGAAGCCG	Made with Primer Blast
Nassau grouper	CO1	Epi_str_For, Epi_str_Rev	58.4 56.4	GCTGGACGGTATATCCCCCT GATACTGGGAGATTGCGGGA	Made with Primer Blast
Mutton snapper	CO1	Lut_ana_For, Lut_ana_Rev	56.4 58.5	CGCTATTCGTTTGAGCCGTC AGATGGCAGGGGGCTTCATA	Made with Primer Blast
Subtropical marine fishes	12S	MiFish-U Forward MiFish-U Reverse	56.6 56.5	GTCGGTAAAACTCGTGCCAGC GTATCACCCCATAGATTAGGGTCAAAC	Miya et al. (2015)
Red hind	12S	RH-F 12S, RH-R 12S	53.8 52.7	GGAACGCTCTGCTTTCTG GGCTACATTCCCTGTCAC	GenBank: AY949437
Nassau grouper	12S	NS-F 12S, NS-R 12S	57.6 56.6	TCTAAAGCACCGCCAAGTCC ACACAATAACTATCCGCCTGGAG	GenBank: AY949433
Yellowfin grouper	12S	YF-F 12S, YF-R 12S	51.2 53.2	GTAATAGGGAATGTAGCCCA GGCCCTTCAGTAAGCAC	GenBank: AY949419
Mutton snapper	12S	MT-F 12S, MT-R 12S	56.3 56.2	CGCCTATATACCACCGTCGT TCCATACGCTACACCTCGAC	GenBank: EF095569