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. 2022 Nov 15;114(2):533–545. doi: 10.1111/cas.15618

FIGURE 3.

FIGURE 3

TUFT1 SUMOylation is necessary for AKT/mTOR activation. (A, B) Evaluating the effects of TUFT1 overexpression on AKT/mTOR signaling activation in AGS and HGC27 cells by immunoblotting. Phospho‐AKT (p‐AKT) indicates AKT activation and 70‐kDa ribosomal S6 kinase (S6K1) is a downstream effector of mTOR. Quantification is shown in (B) (n = 3). (C, D) Evaluating the effects of TUFT1 KO on AKT/mTOR signaling activation in AGS and HGC27 cells by immunoblotting (n = 3). Exogenous TUFT1‐WT or TUFT1‐KR were transiently transfected into the KO cells. Asterisks indicate exogenous TUFT1. Quantification is shown in (D) (n = 3). Data are presented as means ± SEM. P values were determined by unpaired two‐tailed Student's t‐test. ***P < 0.001, **P < 0.01, *P < 0.05; n.s., not significant.