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. 2022 Apr 28;114(2):423–435. doi: 10.1111/cas.15368

FIGURE 4.

FIGURE 4

CCL‐14 is the key secreted molecule from HCC cells in MMP21‐mediated macrophage recruitment. (A) Functional KEGG pathway enrichment from a TCGA‐LIHC data set. (B) The correlation between MMP‐21 and CCL‐14 from a TCGA‐LIHC data set. (C) Protein levels of MMP‐21 and CCL‐14 in HCC tissues. (D) Correlation between MMP‐21 and CCL‐14 for mRNA levels in HCC tissues. (E) IHC analysis of MMP‐21, CCL‐14, and CD68 in HCC tissues. (F) CCL‐14 expression in 7402 cells with increasing MMP‐21. (G) CCL‐14 expression in 7721 cells with decreasing MMP‐21. (H) Knockdown efficiency of CCL‐14 in 7402 cells. (I) Transwell test to determine the macrophage recruitment using 7402 cells with CCL‐14 knockdown. (J) Overexpression efficiency of MMP21 in a CCL14‐knockdown 7402 stable cell line. (K) Macrophage recruitment assessment using the CCL14‐knockdown 7402 stable cell line with MMP‐21 overexpression. *p < 0.05, **p < 0.01; ns, no significance. [Correction added on 21 September 2022, after first online publication: in Figure 4, part G has been replaced with the correct image].