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. 2022 Nov 21;15(2):240–247. doi: 10.1038/s41557-022-01090-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, Normalized fluorescence of Py-PEG₄-Im loaded onto polymersomes and Py-PEG₄-Im dissolved in water. The fluorescence intensity was normalized at the first fluorescence peak (I₁). b,c, Brightfield (left) and fluorescence images (right) of the aqueous dispersion of PEG microparticles (b) and polystyrene microparticles (c) after incubation with Py-PEG₄-Im. d, Snapshot of the loading of Py-PEG₄-Im onto the PEG corona of polymersomes at 10 ns. Py-PEG₄-Im is shown as the space-filling model and PEG corona is shown as the framework model (cyan, C; red, O; blue, N; grey, H). e, Radial distribution function (g(r)) of Py-PEG corona, PEG₄-PEG corona and Im-PEG corona at 8–10 ns. r is the distance between different segments of molecular probe and PEG corona. f, ITC analysis for the binding of molecular probes with polymersomes. The dotted lines represent the fitted plots generated by MicroCal PEAQ-ITC Analysis Software. For Ip, Ph, Na and Py (but not Hy), ΔH < 0 and ΔS > 0. g, Schematic showing the loading of Py-PEG₄-Os onto PEG corona.

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