Table 2.
Genes/proteins involved in the contribution of extracellular polymeric matrix (EPS) components in B. pseudomallei biofilms.
| EPS components | Gene/gene cluster reported to be involved in EPS biosynthesis (Annotation/Description) | Species/isolate | Sequence identity to BP K96243 (% of identity) | Burkholderia pseudomallei K96243 identifier code | Protein Description of Burkholderia pseudomallei K96243 | Reference |
|---|---|---|---|---|---|---|
| Exopolysaccharide | Bcam1330 (putative exopolysaccharide export protein) | Burkholderia cenocepacia J2315 | 79.10 | BPSL2780 | Capsular polysaccharide transport protein | [54,97] |
| Bcam1331 (putative tyrosine kinase protein) | Burkholderia cenocepacia J2315 | - | - | - | [54,97] | |
| Bcam1332 (hypothetical protein) | Burkholderia cenocepacia J2315 | - | - | - | [54,97] | |
| Bcam1333 (putative exopolysaccharide acyltransferase) | Burkholderia cenocepacia J2315 | 73.68 | BPSL3087 | Acyltransferase | [54,97] | |
| Bcam1334 (hypothetical protein) | Burkholderia cenocepacia J2315 | 70.37 | BPSL0610 | Hypothetical protein | [54,97] | |
| Bcam1335 (glycosyltransferase) | Burkholderia cenocepacia J2315 | 71.77 | BPSL0604 | Glycosyltransferase | [54,97] | |
| Bcam1336 (putative exopolysaccharide transporter) | Burkholderia cenocepacia J2315 | 74.91 | BPSL0603 | polysaccharide biosynthesis protein | [54,97] | |
| Bcam1337 (glycosyltransferase) | Burkholderia cenocepacia J2315 | - | - | - | [54,97] | |
| Bcam1338 (glycosyltransferase) | Burkholderia cenocepacia J2315 | - | - | - | [54,97] | |
| Bcam1339 (hypothetical protein) | Burkholderia cenocepacia J2315 | 73.68 | BPSL1233 | Lipoprotein | [54,97] | |
| Bcam1340 (mannose-1-gyanylyltransferase) | Burkholderia cenocepacia J2315 | 83.60 | BPSL0605 | Mannose-1-phosphate guanylyltransferase (manC) | [45,54,97] | |
| Bcam1340 (mannose-1-gyanylyltransferase) | Burkholderia cenocepacia J2315 | 74.61 | BPSS1835 | LPS biosynthesis mannose-1-phosphate guanylyltransferase (BceA) | [54,97] | |
| Bcam1341 (hypothetical protein) | Burkholderia cenocepacia J2315 | 77.67 | BPSL0606 | Hypothetical protein | [54,97] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSL0618 | putative sugar transferase | [45] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSL0619 | putative polysaccharide biosynthesis/export protein | [45] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSL0620 | glycosyl transferase group 1 protein | [45] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSS1649 | sugar-binding protein | [45] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSS1978 | EPS transport-related membrane protein kinase | [45] | |
| Bp 1026b_I2907-Bp1026b_I2927 becA-R | Burkholderia pseudomallei 1026b | NA | BPSL0603-BPSL0620 | Exopolysaccharide gene cluster | [96] | |
| Bp1026b-I0648 wbiA | Burkholderia pseudomallei 1026b | 99.1 | BPSL2671 | Glycosyltransferase family protein | [54,96] | |
| Bp1026b-I0649 wbiA | Burkholderia pseudomallei 1026b | 100 | BPSL2670 | UDP-glucose-4-epimerase | [54,96] | |
| bpsI | Burkholderia pseudomallei K96243 | NA | BPSS0885 | acyl homoserine lactone (AHL) | [93] | |
| rpoS | Burkholderia pseudomallei K96243 | NA | BPSL1505 | RNA polymerase sigma factor | [93] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSL1366 | polyphosphate kinase | [93] | |
| wcbK | Burkholderia pseudomallei K96243 | NA | BPSL2729 | UTP glucose-1-phosphate | [93] | |
| eDNA | Bcal1585 (histone like protein) (hupb) | Burkholderia cenocepacia J2315 | 76.98 | BPSL0004 | DNA-binding protein HU-alpha | [54,122] |
| Bcal3530 (histone like protein) (hupA) | Burkholderia cenocepacia J2315 | 93.45 | BPSL0004 | DNA-binding protein HU-alpha | [54,122] | |
| Bcal1487 (integration host factor alpha) | Burkholderia cenocepacia J2315 | 88.04 | BPSL1939 | integration host factor alpha | [54,122] | |
| Bcal2949 (integration host factor beta) | Burkholderia cenocepacia J2315 | 89.56 | BPSL2514 | integration host factor beta | [54,122] | |
| BPSL1887 (transcriptional regulatory protein) | Burkholderia pseudomallei K96243 | NA | BPSL1887 | sigma-54 related transcriptional regulatory protein | [110] | |
| Proteins | - | Burkholderia pseudomallei K96243 | NA | BPSS0093 | outer membrane usher protein | [45] |
| - | Burkholderia pseudomallei K96243 | NA | BPSL1800 | outer membrane usher protein | [45] | |
| bceF | Burkholderia pseudomallei K96243 | NA | BPSS1830 | Tyrosine kinase | [54] | |
| AK34_RS27645 (Alginate lyase) | Burkholderia dolosa AU0158 | 85.42 | BPSL3363 | Hypothetical protein | [54] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSL0782 | Type 4 Pili 1 | [130] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSL1821 | Type 4 Pili 2 | [130] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSL1899 | Type 4 Pili 3 | [130] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSL2752 | Type 4 Pili 4 | [130] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSL2756 | Type 4 Pili 4 | [130] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSL3008 | Type 4 Pili 5 | [130] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSL3170 | Type 4 Pili 6 | [130] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSS1593 | Type 4 Pili 7 | [130] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSS1595 | Type 4 Pili 7 | [130] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSS2185 | Type 4 Pili 8 | [130] | |
| - | Burkholderia pseudomallei K96243 | NA | BPSS2186 | Type 4 Pili 8 | [130] |
*NA-Not applicable