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. 2023 Jan 23;16:1076765. doi: 10.3389/fnbeh.2022.1076765

Table 1.

Summary of statistical parameters.

Test Data structure Type of test Description of analysis Test value p-value Effect size
Open Field—Acute Deprivation
Center Time Non-normal Wilcoxon rank-sum test Effect of sex W = 657 0.919 r = 0.0126
Non-normal Kruskal-Wallis test: males Effect of condition χ2(2) = 0.893 0.64 η2(H) = −0.033
Non-normal Kruskal-Wallis test: females Effect of condition χ2(2) = 1.9 0.387 η2(H) = −0.003
Play—Acute Deprivation
Total Play Normal distribution Two-way ANOVA Main effect: sex F(1,36) = 4.929 0.036 η2(H) = −0.101
Normal distribution Two-way ANOVA Main effect: condition F(1,36) = 5.358 0.026 η2(H) = −0.11
Normal distribution Two-way ANOVA Interaction: sex × condition F(1,36) = 2.357 0.134 η2(H) = 0.048
Elevated Plus Maze—SDB
Time in Open Arms (%) Normal distribution Two-way ANOVA Main effect: sex F(1,70) = 2.835 0.097 η2 = 0.038
Normal distribution Two-way ANOVA Main effect: condition F(2,70) = 1.330 0.271 η2 = 0.037
Normal distribution Two-way ANOVA Interaction: sex × condition F(2,70) = 0.107 0.899 η2 = 0.003
Distance Traveled Normal distribution Two-way ANOVA Main effect: sex F(1,70) = 4.704 0.034 η2 = 0.065
Normal distribution Two-way ANOVA Main effect: condition F(2,70) = 0.183 0.833 η2 = 0.005
Normal distribution Two-way ANOVA Interaction: sex × condition F(2,70) = 0.912 0.406 η2 = 0.025
Elevated Plus Maze—LDB
Time in Open Arms (%) Normal distribution Two-way ANOVA Main effect: sex F(1,62) = 7.654 0.007 η2 = 0.106
Normal distribution Two-way ANOVA Main effect: condition F(2,62) = 3.038 0.055 η2 = 0.089
Normal distribution Two-way ANOVA Interaction: sex × condition F(2,62) = 0.149 0.862 η2 = 0.005
Distance Traveled Normal distribution Two-way ANOVA Main effect: sex F(1,62) = 8.595 0.005 η2 = 0.113
Normal distribution Two-way ANOVA Main effect: condition F(2,62) = 10.326 <0.001 η2 = 0.250
Two-way ANOVA: Tukey post-hoc CTRL vs. BARR n/a <0.001 d = 1.655
Two-way ANOVA: Tukey post-hoc CTRL vs. ISO n/a 0.544 d = 0.298
Two-way ANOVA: Tukey post-hoc BARR vs. ISO n/a 0.003 d = 0.937
Normal distribution Two-way ANOVA Interaction: sex × condition F(2,62) = 0.239 0.788 η2 = 0.008
Open Field Test—LDB
Center Time Non-normal Wilcoxon rank-sum test Effect of sex W = 1,080.5 <0.001 r = 0.428
Non-normal Kruskal-Wallis test: males Effect of condition χ2(2) = 0.024 0.988 d = −0.055
Non-normal Kruskal-Wallis test: females Effect of condition χ2(2) = 0.234 0.89 d = −0.052
Line Crossings Normal distribution Two-way ANOVA Main effect: sex F(1,70) = 19.673 <0.001 η2 = 0.221
Normal distribution Two-way ANOVA Main effect: condition F(2,70) = 4.649 0.013 η2 = 0.117
Two-way ANOVA: Tukey post-hoc CTRL vs. BARR n/a 0.01 d = 0.709
Two-way ANOVA: Tukey post-hoc CTRL vs. ISO n/a 0.117 d = 0.509
Two-way ANOVA: Tukey post-hoc BARR vs. ISO n/a 0.512 d = 0.311
Normal distribution Two-way ANOVA Interaction: sex × condition F(2,70) = 0.777 0.464 η2 = 0.022
Novel Object—SDB
Discrimination Ratio Normal distribution One sample t-test Male CTRL vs. 0 t(11) = 3.369 0.006 d = 0.972
Normal distribution One sample t-test Male BARR vs. 0 t(13) = 4.537 <0.001 d = 1.213
Normal distribution One sample t-test Male ISO vs. 0 t(12) = 3.437 0.005 d = 0.953
Normal distribution One sample t-test Female CTRL vs. 0 t(13) = 2.822 0.014 d = 0.754
Normal distribution One sample t-test Female BARR vs. 0 t(13) = 3.322 0.006 d = 0.888
Normal distribution One sample t-test Female ISO vs. 0 t(10) = 3.932 0.003 d = 1.186
Normal distribution Two-way ANOVA Main effect: sex F(1,72) = 0.421 0.519 η2 = 0.008
Normal distribution Two-way ANOVA Main effect: condition F(2,72) = 1.334 0.27 η2 = 0.036
Normal distribution Two-way ANOVA Interaction: sex × condition F(2,72) = 0.634 0.533 η2 = 0.017
Novel Object—LDB
Discrimination Ratio Normal distribution One sample t-test Male CTRL vs. 0 t(10) = 2.631 0.025 d = 0.793
Normal distribution One sample t-test Male BARR vs. 0 t(8) = 3.806 0.005 d = 1.269
Normal distribution One sample t-test Male ISO vs. 0 t(14) = 3.943 0.001 d = 1.018
Normal distribution One sample t-test Female CTRL vs. 0 t(10) = 4.947 <0.001 d = 1.492
Normal distribution One sample t-test Female BARR vs. 0 t(11) = 3.541 0.005 d = 1.022
Normal distribution One sample t-test Female ISO vs. 0 t(12) = 0.001 0.003 d = 1.165
Normal distribution Two-way ANOVA Main effect: sex F(1,65) = 0.756 0.388 η2 = 0.013
Normal distribution Two-way ANOVA Main effect: condition F(2,65) = 0.416 0.661 η2 = 0.013
Normal distribution Two-way ANOVA Interaction: sex × condition F(2,65) = 0.723 0.489 η2 = 0.022
Female Sex Behavior—SDB
Lordosis Quotient Non-normal Kruskal-Wallis test Effect of condition χ2(2) = 3.257 0.196 η2(H) = 0.034
Proceptive Behaviors Normal distribution One-way ANOVA Effect of condition F(2,37) = 0.137 0.873 η2 = 0.007
Female Sex Behavior—LDB
Lordosis Quotient Non-normal Kruskal-Wallis test Effect of condition χ2(2) = 3.52 0.172 η2(H) = 0.056
Proceptive Behaviors Non-normal Kruskal-Wallis test Effect of condition χ2(2) = 1.61 0.447 η2(H) = −0.014
Male Sex Behavior—SDB
Average Number of Mounts Normal distribution One-way ANOVA Effect of condition F(2,35) = 2.786 0.075 0.216
Average Number of Intromissions Normal distribution One-way ANOVA Effect of condition F(2,35) = 2.165 0.13 0.137
Average Mount Rate Normal distribution One-way ANOVA Effect of condition F(2,35) = 4.818 0.014 0.11
Kruskal-Wallis: Fisher’s LSD post-hoc CTRL vs. BARR n/a 0.03 d = 0.618
Kruskal-Wallis: Fisher’s LSD post-hoc CTRL vs. ISO n/a 0.005 d = 0.981
Kruskal-Wallis: Fisher’s LSD post-hoc BARR vs. ISO n/a 0.407 d = 0.221
Percent that Ejaculated Non-normal Chi-square goodness of fit test % ejaculated vs. 90% - CTRL χ2 = 0.818 0.366 φ= 0.273
Chi-square goodness of fit test % ejaculated vs. 90% - BARR χ2 = 0.127 0.722 φ= 0.095
Chi-square goodness of fit test % ejaculated vs. 90% - ISO χ2 = 0.419 0.518 φ= 0.18
Male Sex Behavior—LDB
Average Number of Mounts Normal distribution One-way ANOVA Effect of condition F(2,33) = 3.949 0.029 η2 = 0.193
One-way ANOVA: Fisher’s LSD post-hoc CTRL vs. BARR n/a 0.03 d = 0.654
One-way ANOVA: Fisher’s LSD post-hoc CTRL vs. ISO n/a 0.014 d = 0.626
One-way ANOVA: Fisher’s LSD post-hoc BARR vs. ISO n/a 0.767 d = 0.06
Average Number of Intromissions Non-normal Kruskal-Wallis test Effect of condition χ2(2) = 8.304 0.016 η2(H) = 0.191
Kruskal-Wallis: Fisher’s LSD post-hoc CTRL vs. BARR n/a 0.026 d = 0.796
Kruskal-Wallis: Fisher’s LSD post-hoc CTRL vs. ISO n/a 0.012 d = 0.906
Kruskal-Wallis: Fisher’s LSD post-hoc BARR vs. ISO n/a 0.763 d = 0.182
Average Mount Rate Non-normal Kruskal-Wallis test Effect of condition χ2(2) = 3.779 0.151 η2(H) = 0.054
Percent that Ejaculated Non-normal Chi-square goodness of fit test % ejaculated vs. 90% - CTRL χ2 = 0.056 0.814 φ = 0.084
Chi-square goodness of fit test % ejaculated vs. 90% - BARR χ2 = 10 0.002 φ = 1
Chi-square goodness of fit test % ejaculated vs. 90% - ISO χ2 = 11.701 <0.001 φ = 0.949
Social Preference—SDB
Percent of Time Near Social Box Normal distribution One sample t-test Male CTRL vs. 50% t(11) = 8.363 <0.001 d = 2.414
Normal distribution One sample t-test Male BARR vs. 50% t(13) = 19.047 <0.001 d = 5.091
Normal distribution One sample t-test Male ISO vs. 50% t(12) = 6.702 <0.001 d = 1.859
Normal distribution One sample t-test Female CTRL vs. 50% t(13) = 10.423 <0.001 d = 2.786
Normal distribution One sample t-test Female BARR vs. 50% t(13) = 7.674 <0.001 d = 2.051
Normal distribution One sample t-test Female ISO vs. 50% t(11) = 6.631 <0.001 d = 1.914
Normal distribution Two-way ANOVA Main effect: sex F(1,73) = 18.582 <0.001 η2 = 0.142
Normal distribution Two-way ANOVA Main effect: condition F(2,73) = 8.168 <0.001 η2 = 0.126
Normal distribution Two-way ANOVA Interaction: sex × condition F(2,73) = 10.672 <0.001 η2 = 0.165
Two-way ANOVA: Tukey post-hoc Male CTRL vs. Female CTRL n/a 0.778 d = 0.579
Two-way ANOVA: Tukey post-hoc Female BARR vs. Female CTRL n/a 1 d = 0.006
Two-way ANOVA: Tukey post-hoc Male BARR vs. Female CTRL n/a <0.001 d = 2.939
Two-way ANOVA: Tukey post-hoc Female ISO vs. Female CTRL n/a 0.99 d = 0.248
Two-way ANOVA: Tukey post-hoc Male ISO vs. Female CTRL n/a 0.999 d = 0.147
Two-way ANOVA: Tukey post-hoc Female BARR vs. Male CTRL n/a 0.786 d = 0.501
Two-way ANOVA: Tukey post-hoc Male BARR vs. Male CTRL n/a <0.001 d = 1.911
Two-way ANOVA: Tukey post-hoc Female ISO vs. Male CTRL n/a 0.984 d = 0.247
Two-way ANOVA: Tukey post-hoc Male ISO vs. Male CTRL n/a 0.936 d = 0.247
Two-way ANOVA: Tukey post-hoc Male BARR vs. Female BARR n/a <0.001 d = 2.515
Two-way ANOVA: Tukey post-hoc Female ISO vs. Female BARR n/a 0.991 d = 0.215
Two-way ANOVA: Tukey post-hoc Male ISO vs. Female BARR n/a 0.999 d = 0.126
Two-way ANOVA: Tukey post-hoc Female ISO vs. Male BARR n/a <0.001 d = 2.056
Two-way ANOVA: Tukey post-hoc Male ISO vs. Male BARR n/a <0.001 d = 2.156
Two-way ANOVA: Tukey post-hoc Male ISO vs. Female ISO n/a 1 d = 0.082
Ratio Time Near Social Box Non-normal Wilcoxon rank-sum test Effect of sex W = 449 0.001 r = 0.365
Non-normal Kruskal-Wallis test: males Effect of condition χ2(2) = 19.374 <0.001 η2(H) = 0.483
Kruskal-Wallis: Wilcoxon post-hoc Male CTRL vs. BARR W = 15 <0.001 r = 0.696
Kruskal-Wallis: Wilcoxon post-hoc Male CTRL vs. ISO W = 98 0.295 r = 0.218
Kruskal-Wallis: Wilcoxon post-hoc Male BARR vs. ISO W = 170 <0.001 r = 0.738
Non-normal Kruskal-Wallis test: females Effect of condition χ2(2) = 0.557 0.757 η2(H) = −0.039
Social Preference—LDB
Percent of Time Near Social Box Normal distribution One sample t-test Male CTRL vs. 50% t(11) = 6.639 <0.001 d = 1.917
Normal distribution One sample t-test Male BARR vs. 50% t(11) = 6.794 <0.001 d = 1.961
Normal distribution One sample t-test Male ISO vs. 50% t(10) = 3.837 0.003 d = 1.157
Normal distribution One sample t-test Female CTRL vs. 50% t(11) = 5.178 <0.001 d = 1.495
Normal distribution One sample t-test Female BARR vs. 50% t(11) = 5.547 <0.001 d = 1.601
Normal distribution One sample t-test Female ISO vs. 50% t(12) = 2.520 0.027 d = 0.699
Normal distribution Two-way ANOVA Main effect: sex F(1,66) = 9.471 0.003 η2 = 0.121
Normal distribution Two-way ANOVA Main effect: condition F(2,66) = 1.192 0.31 η2 = 0.03
Normal distribution Two-way ANOVA Interaction: sex × condition F(2,66) = 0.239 0.788 η2 = 0.006
Ratio Time Near Social Box Non-normal Wilcoxon rank-sum test Effect of sex W = 354 <0.001 r = 0.390
Non-normal Kruskal-Wallis test: males Effect of condition χ2(2) = 0.134 0.935 η2(H) = −0.058
Non-normal Kruskal-Wallis test: females Effect of condition χ2(2) = 2.51 0.285 η2(H) = 0.015
Sex Preference—SDB
Percent Time Near Opposite-Sex Box Normal distribution One sample t-test Male CTRL vs. 50% t(11) = 3.953 0.002 d = 1.141
Normal distribution One sample t-test Male BARR vs. 50% t(13) = 4.009 0.001 d = 1.071
Normal distribution One sample t-test Male ISO vs. 50% t(12) = 2.423 0.032 d = 0.672
Normal distribution One sample t-test Female CTRL vs. 50% t(13) = 2.355 0.035 d = 0.629
Normal distribution One sample t-test Female BARR vs. 50% t(13) = 3.007 0.01 d = 0.804
Normal distribution One sample t-test Female ISO vs. 50% t(11) = 3.558 0.004 d = 1.027
Normal distribution Two-way ANOVA Main effect: sex F(1,73) = 0.037 0.849 η2 = 0.001
Normal distribution Two-way ANOVA Main effect: condition F(2,73) = 0.559 0.574 η2 = 0.015
Normal distribution Two-way ANOVA Interaction: sex × condition F(2,73) = 0.996 0.374 η2 = 0.026
Ratio Time Near Opposite-Sex Box Non-normal Wilcoxon rank-sum test Effect of sex W =795 0.887 r = 0.017
Non-normal Kruskal-Wallis test: males Effect of condition χ2(2) = 3.334 0.189 η2(H) = 0.037
Non-normal Kruskal-Wallis test: females Effect of condition χ2(2) = 0.017 0.992 η2(H) = −0.054
Sex Preference—LDB
Percent Time Near Opposite-Sex Box Normal distribution One sample t-test Male CTRL vs. 50% t(11) = 3.313 0.007 d = 0.956
Normal distribution One sample t-test Male BARR vs. 50% t(11) = 1.966 0.075 d = 0.567
Normal distribution One sample t-test Male ISO vs. 50% t(11) = 2.339 0.04 d = 0.675
Normal distribution One sample t-test Female CTRL vs. 50% t(11) = 4.881 <0.001 d = 1.409
Normal distribution One sample t-test Female BARR vs. 50% t(11) = 1.557 0.148 d = 0.449
Normal distribution One sample t-test Female ISO vs. 50% t(12) = 4.034 0.002 d = 1.119
Normal distribution Two-way ANOVA Main effect: sex F(1,67) = 0.741 0.392 η2 = 0.011
Normal distribution Two-way ANOVA Main effect: condition F(2,67) = 0.811 0.449 η2 = 0.023
Normal distribution Two-way ANOVA Interaction: sex × condition F(2,67) = 1.167 0.317 η2 = 0.033
Ratio Time Near Opposite-Sex Non-normal Wilcoxon rank-sum test Effect of sex W = 595 0.437 r = 0.092
Non-normal Kruskal-Wallis test: males Effect of condition χ2(2) = 1.5 0.472 η2(H) = −0.015
Non-normal Kruskal-Wallis test: females Effect of condition χ2(2) = 5.059 0.08 η2(H) = 0.09
Interaction Time Normal distribution Paired t-test Male CTRL-Train vs. Test t(12) = 2.336 0.038 d = 0.592
Normal distribution Paired t-test Male BARR-Train vs. Test t(11) = 1.892 0.085 d = 0.518
Normal distribution Paired t-test Male ISO-Train vs. Test t(12) = 2.491 0.028 d = 0.746
Normal distribution Paired t-test Female CTRL-Train vs. Test t(9) = 4.316 0.002 d = 1.276
Normal distribution Paired t-test Female BARR-Train vs. Test t(8) = 2.692 0.027 d = 1.055
Normal distribution Paired t-test Female ISO-Train vs. Test t(8) = 4.791 0.001 d = 1.455
Ratio of Investigation Duration Non-normal Wilcoxon rank-sum test Effect of sex W = 253 <0.001 r = 0.446
Non-normal Kruskal-Wallis test: males Effect of condition χ2(2) = 0.03 0.985 η2(H) = −0.056
Non-normal Kruskal-Wallis test: females Effect of condition χ2(2) = 0.22 0.896 η2(H) = −0.072
Social Recognition-LDB
Interaction Time Normal distribution Paired t-test Male CTRL-Train vs. Test t(11) = 2.653 0.022 d = 0.713
Normal distribution Paired t-test Male BARR-Train vs. Test t(11) = 2.425 0.033 d = 0.707
Normal distribution Paired t-test Male ISO-Train vs. Test t(11) = 3.019 0.012 d = 0.629
Normal distribution Paired t-test Female CTRL-Train vs. Test t(11) = 6.737 <0.001 d = 1.861
Normal distribution Paired t-test Female BARR-Train vs. Test t(11) = 10.557 <0.001 d = 3.492
Normal distribution Paired t-test Female ISO-Train vs. Test t(12) = 5.839 <0.001 d = 1.477
Ratio of Investigation Duration Non-normal Wilcoxon rank-sum test Effect of sex W = 110 <0.001 r = 0.718
Non-normal Kruskal-Wallis test: males Effect of condition χ2(2) = 0.126 0.939 η2(H) = −0.057
Non-normal Kruskal-Wallis test: females Effect of condition χ2(2) = 3.552 0.169 η2(H) = 0.046
Resident Intruder Assay-LDB
Average Number of Keep Downs Normal distribution One-way ANOVA Effect of condition F(2,27) = 1.773 0.189 η2 = 0.116
Average Number of Lateral Threats Non-normal Kruskal-Wallis test Effect of condition χ2(2) = 4.08 0.13 η2(H) = 0.077
Average Number of Upright Postures Non-normal Kruskal-Wallis test Effect of condition χ2(2) = 4.856 0.088 η2(H) = 0.106
Average Number of Clinch Attacks Non-normal Kruskal-Wallis test Effect of condition χ2(2) = 0.799 0.671 η2(H) = −0.045
Average Number of Aggressive Behaviors (Total) Normal distribution One-way ANOVA Effect of condition F(2,27) = 4.815 0.016 η2 = 0.263
One-way ANOVA: Fisher’s LSD post-hoc CTRL vs. BARR n/a 0.056 d = 0.863
One-way ANOVA: Fisher’s LSD post-hoc CTRL vs. ISO n/a 0.005 d = 1.481
One-way ANOVA: Fisher’s LSD post-hoc BARR vs. ISO n/a 0.30 d = 0.456
Empathy/Prosocial Helping Behavior
Average Mean Latency to Release Cagemate—Days 1–6 Normal distribution Two-way ANOVA Main effect: sex F(1,15) = 4.757 0.046 η2 = 0.217
Normal distribution Two-way ANOVA Main effect: condition F(1,15) = 6.049 0.027 η2 = 0.287
Normal distribution Two-way ANOVA Interaction: sex × condition F(1,15) = 0.157 0.697 η2 = 0.01
Average Mean Latency to Release Cagemate—Days 7–12 Normal distribution Two-way ANOVA Main effect: sex F(1,15) = 4.028 0.063 η2 = 0.217
Normal distribution Two-way ANOVA Main effect: condition F(1,15) = 0.344 0.566 η2 = 0.022
Normal distribution Two-way ANOVA Interaction: sex × condition F(1,15) = 0.99 0.336 η2 = 0.062
Factor Analysis–SDB
Factor Scores—Anxiety Normal distribution One-way ANOVA Effect of condition F(2,20) = 0.439 0.651 η2 = 0.042
Factor Scores—Salience Normal distribution One-way ANOVA Effect of condition F(2,20) = 4.029 0.034 η2 = 0.287
One-way ANOVA: Fisher’s LSD post-hoc CTRL vs. BARR n/a 0.0285 d = 1.521
One-way ANOVA: Fisher’s LSD post-hoc CTRL vs. ISO n/a 0.015 d = 1.33
One-way ANOVA: Fisher’s LSD post-hoc BARR vs. ISO n/a 0.797 d = 0.119
Factor Scores—Sociosexual Behavior Normal distribution One-way ANOVA Effect of condition F(2,20) = 3.674 0.044 η2 = 0.269
One-way ANOVA: Fisher’s LSD post-hoc CTRL vs. BARR n/a 0.019 d = 1.265
One-way ANOVA: Fisher’s LSD post-hoc CTRL vs. ISO n/a 0.447 d = 0.55
One-way ANOVA: Fisher’s LSD post-hoc BARR vs. ISO n/a 0.059 d = 0.877
Factor Scores—Anxiety I Normal distribution One-way ANOVA Effect of condition F(2,20) = 0.537 0.593 η2 = 0.051
Factor Scores—Anxiety II Normal distribution One-way ANOVA Effect of condition F(2,20) = 3.306 0.058 η2 = 0.248
Factor Scores—Sociosexual Behavior Normal distribution One-way ANOVA Effect of condition F(2,20) = 4.196 0.03 η2 = 0.296
One-way ANOVA: Fisher’s LSD post-hoc CTRL vs. BARR n/a 0.009 d = 1.706
One-way ANOVA: Fisher’s LSD post-hoc CTRL vs. ISO n/a 0.167 d = 0.746
One-way ANOVA: Fisher’s LSD post-hoc BARR vs. ISO n/a 0.147 d = 0.683