Figure 1.

Transwell migration assay with normal Brca1 KO (knockout) murine mammary organoids. (A) Overall scheme to test migratory properties of organoid cultures. Mammary-derived organoid cultures can either be dissociated into single cells or plated as whole organoids, into transwell chambers. (B) Normal Brca1 KO mammary-derived organoid cultures, either bearing 1 copy or total loss of the p53 gene, were tested for their mobility capacity in transwell chambers, indicating differential migratory capacity based on p53 genotype. Scale bar: 100 µm.