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. 2023 Feb 3;247(24):2201–2212. doi: 10.1177/15353702231151241

Figure 5.

Figure 5.

The presence of a second DENV-4 component confers induction of markers that are key in dendritic cell (DC) migration. (A, B) Monocyte-derived DCs (MDDCs) were infected with mock, trivalent vaccine, tetravalent vaccine or DEN-4Δ30 × 2 formulation. Multiplex ELISA was performed as in previous experiments to quantify the levels of MCP-1 and RANTES secretion, respectively, to the culture supernatant at 6, 24, and 48 hpi. (C) Using mass cytometry by time-of-flight (CyTOF) analysis for each mock, trivalent vaccine, tetravalent vaccine, or DEN-4Δ30 × 2 formulation sample. The percent of cells expressing CCR7 was quantified. (D) CyTOF depicting the median expression of CD40 of total cell populations is shown. Black asterisks represent statistical significance by the two-way analysis of variance (ANOVA), with adjustment for multiple comparisons and the Bonferroni correction (*P ⩽ 0.05, **P ⩽ 0.01, **P ⩽ 0.001). (A color version of this figure is available in the online journal.)