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. Author manuscript; available in PMC: 2023 Feb 6.
Published in final edited form as: Stem Cell Res. 2022 Nov 30;66:102987. doi: 10.1016/j.scr.2022.102987

Table 1.

Characterization and validation.

classification Test Result Data
Morphology Photography Bright field Normal Fig. 1D
Quantitative analysis (RT-qPCR) mRNA expression of NANOG and SOX2 Fig. 1G
Genotype Karyotype (G-banding) and resolution [mandatory] Normal Karyotype 46XX Resolution 5 Mb Fig. 1C
Identity Microsatellite PCR (mPCR) OR N/A N/A
STR analysis [mandatory] Matched 27 allelic polymorphisms across 15 STR loci Available with the authors
Mutation analysis (IF APPLICABLE) Sequencing Heterozygous Fig. 1B
Southern Blot OR WGS N/A N/A
Microbiology and virology Mycoplasma Luminescence:Negative Supplementary
Donor screening (OPTIONAL) HIV 1 + 2 Hepatitis B, Hepatitis C N/A N/A
Genotype additional info (OPTIONAL) Blood group genotyping N/A N/A
HLA tissue typing N/A N/A