Fig. 2.

Inhibition of FOXO1 reduced colony number. (A) U87MG cells were treated with AS1842856; colony number was not significantly impacted. (B, C) Indicated colon cancer (HCT116 or SW480) cell lines were treated with FOXO1 inhibitor AS1842856 for 5 days and subsequently stained with crystal violet. AS1842856 decreased colony formation in these cell lines. (D–F) Treatment with another FOXO1 inhibitor AS1708727 in BT549, MDA‐MB‐468, and LN18 cells also led to reduced colony formation. The results are representative of three independent experiments. Values in bar graphs are the mean with SEM. * denotes significantly different by the Tukey test compared with indicated samples (P < 0.05).