Fig. 4.

FOXO Factor RNAi Treatment led to the induction of apoptotic genes. (A, B) Indicated cell lines were treated with FOXO1 RNAi or control (MDA‐MB‐468 72 h post‐transfection) or (BT549 18 h post‐transfection) and examined for changes in gene expression by qRT‐PCR using TUBB as the reference gene. We found that FOXO1 RNAi treatment‐induced FAS in MDA‐MB‐468 cells. FOXO1 RNAi samples had increased BIM and decreased FOXO3 and FOXO4 in BT549 cells. (C, D) FOXO3 or FOXO4 RNAi was examined in BT549 cells. (E) 5AZA treatment (3 μm for 48 h) led to the induction of FAS, and BIM in BT549 cells. The results are representative of three independent experiments. Values in bar graphs are the mean with SEM. * denotes significantly different by the Tukey test compared with the control (P < 0.05).