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. 2023 Feb 1;17:283–295. doi: 10.2147/DDDT.S392813

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© 2023 Yin et al.

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VCM promoted the accumulation of LIP and ROS and regulated the expression of ferroptosis-related proteins ACSL4, PTGS2, GPX4 and SLC7A11 in HK-2 cells. (A) ELISA results revealing the contents of ROS, GSH and LIP in HK-2 cells. We found that the contents of ROS and LIP increased, while that of GSH decreased in VCM-treated HK-2 cells. Furthermore, Fer-1 co-treatment inhibited the accumulation of LIP and ROS and restored the content of GSH partly in VCM-treated HK-2 cells. Expression levels of ACSL4, PTGS2, GPX4 and SLC7A11 in HK-2 cells measured by (B) qRT-PCR analysis, (C) Western blotting analysis and (D) quantification of Western blotting results. The findings exhibited that VCM induced the expression of ACSL4 and PTGS2 while reduced the expression of GPX and SLC7A11 at the mRNA and protein levels in HK-2 cells. In addition, Fer-1 co-treatment decreased the expression of ACSL4 and PTGS2 and restored the expression of GPX and SLC7A11 at the mRNA and protein levels in VCM-treated HK-2 cells. *P<0.05, **P<0.01, ***P<0.001.