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. 2022 Nov 28;24(2):e55778. doi: 10.15252/embr.202255778

Figure 2. M1AP forms discrete foci on the chromosome axes in spermatocytes.

Figure 2

  1. Western blot analysis of M1AP expression in various tissues from adult wild‐type (WT) mice. GAPDH served as the loading control.
  2. Western blot analysis of M1AP expression in testes from mice at different days postpartum (dpp). GAPDH served as the loading control.
  3. Immunofluorescence staining of M1AP (green) and SYCP3 (red) on spermatocyte spreads of WT or Spo11 −/− mice. Scale bar, 10 μm.
  4. Quantification of M1AP foci in WT spermatocytes at the indicated stages. L, leptotene; E/MZ, early/mid zygotene; LZ, late zygotene; EP, early pachytene; M/LP, mid/late pachytene; Z‐like, zygotene‐like. Data are presented as the mean ± SEM. n shows the number of spermatocytes analyzed from three biological replicates.