Figure 7. M1AP colocalizes with TEX11 and facilitates TEX11 recruitment at the recombination intermediates.

1. Immunofluorescence staining of M1AP (green) and TEX11 (red) on the spermatocyte spreads of WT mice.
2. Ratios of M1AP‐TEX11 co‐localizing foci to the M1AP total foci or TEX11 total foci at the indicated meiotic prophase substages. Each dot represents the ratio calculated from one nucleus.
3. Immunofluorescence staining of M1AP (green) and DMC1 (red) on the spermatocyte spreads of WT mice.
4. Ratios of M1AP‐DMC1 co‐localizing foci to the M1AP total foci or DMC1 total foci at the indicated meiotic prophase substages. Each dot represents the ratio calculated from one nucleus.
5. Immunofluorescence staining of M1AP (green) and RPA2 (red) on the spermatocyte spreads of WT mice.
6. Ratios of M1AP‐RPA2 co‐localizing foci to the M1AP total foci or RPA2 total foci at the indicated meiotic prophase substages. Each dot represents the ratio calculated from one nucleus.
7. Immunofluorescence staining of M1AP (green) and SYCP3 (red) on spermatocyte spreads of control, Dmc1 ^−/− or Spo16 ^−/− mice.
8. Immunofluorescence staining of TEX11 (green) and SYCP3 (red) on the spermatocyte spreads of control and M1ap ^KI/KI testes.
9. The mean number of TEX11 foci per cell in control and M1ap ^KI/KI spermatocytes.
10. Frequencies of nuclei with TEX11 foci detected at the PAR in spread early pachytene spermatocytes with touching XY chromosomes.
11. Schematic diagram showing a proposed model in which M1AP acts as a partner of the ZZS complex in meiotic recombination in mouse meiotic prophase I. M1AP is recruited by SPO16 and facilitates TEX11 recruitment to stabilize recombination intermediates.

Data information: (A, C, E, G, and H) Scale bars, 10 μm. (B, D, F, I, and J) Data are presented as the mean ± SEM. n shows the number of cells scored from at least two biological replicates. (I and J) ****P < 0.0001; NS, not significant; two‐tailed Student's t‐test.