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. 2022 Nov 23;24(2):e54006. doi: 10.15252/embr.202154006

Figure EV3. Mdivi1 impairs the mitochondrial accumulation of Parkin and the fusion of the autophagosome with lysosomes in CSCs.

Figure EV3

  1. CSCs with or without Mdivi1 treatment were analyzed to detect Parkin protein levels in mitochondrial fractions and whole cells. Representative from six patients.
  2. Mitochondrial accumulation of Parkin protein in CSCs +/− Mdivi1 treatment. mean ± SEM from six patients.
  3. CSCs were transfected with mCherry‐GFP‐LC3 and treated +/− Mdivi1. Lysosomal fusion with such LC3 protein eradicates GFP, leaving red staining of LC3. Vice versa, strong yellow staining in Mdivi1‐treated CSCs indicates impaired autophagosome fusion with the lysosome. Scale bar, 5μm. Representative from six patients.
  4. Relative intensities of mCherry‐GFP‐LC3 in CSCs +/− Mdivi1 treatment. Collective data (mean ± SEM) from six patients.

Data information: Paired t‐test, **P < 0.01, ***P < 0.001.

Source data are available online for this figure.