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. 2022 Nov 23;24(2):e54006. doi: 10.15252/embr.202154006

Figure 6. TLR9‐Notch1 drives AMPKhigh to promote mitochondrial metabolism in CSCs.

Figure 6

  1. CSCs stimulated with or without CpG ODN (10 μg/ml) were analyzed for expression of phosphor‐AMPKα. Representative and mean ± SEM from six patients.
  2. CSCs were stimulated with CpG ODN (10 μg/ml) in the presence or absence of DAPT (10 μM), and analyzed for phosphor‐AMPKα. Mean ± SEM from six patients.
  3. CSCs transfected with Notch1 shRNA or control were stimulated with CpG ODN (10 μg/ml) and analyzed for phosphor‐AMPKα. Mean ± SEM from six patients.
  4. CSCs were treated with DAPT (10 μM) in the presence or absence of A769662 (10 μM), and analyzed for mitochondrial OCR. Ctrl represents CSCs without any treatment. Mean ± SEM from six patients.
  5. CSCs were stimulated with or without CpG ODN (10 μg/ml) and analyzed for mitochondrial OCR. Mean ± SEM from six patients.
  6. CSCs were stimulated with CpG ODN (10 μg/ml) in the presence or absence of Compound C (10 μM) and analyzed for mitochondrial OCR. Mean ± SEM from six patients.
  7. CSCs were transfected with two different AMPKα shRNA or the control, stimulated with CpG ODN (10 μg/ml), and analyzed for mitochondrial OCR. Mean ± SEM from six patients.

Data information: Paired t‐test with Bonferroni method and ANOVA with Turkey's method (D), **P < 0.01, ***P < 0.001, ****P < 0.0001.

Source data are available online for this figure.