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. 2022 Dec 12;24(2):e55472. doi: 10.15252/embr.202255472

Figure EV3. TFEB‐S401 phosphorylation induced by NaAsO2 and LPS treatments is absent in S401A knock‐in THP1 cells. Related to Fig 3 .

Figure EV3

  1. Flowchart indicating the different steps followed to differentiate primary human monocytes into macrophage cells.
  2. Immunoblot analysis of protein lysates from primary human monocytes undifferentiated (Naïve) or GM‐CSF‐differentiated primary macrophages. Samples are from three independent experiments.
  3. Immunoblot analysis of protein lysates from primary human macrophages incubated with 1 μg/ml LPS for the indicated times. Immunoblots are representative of at least four independent experiments.
  4. Quantification of immunoblot data shown in (C). Data are presented as mean ± SD using one‐way ANOVA (unpaired) followed by Dunnett's multiple comparisons test, **P < 0.01, ***P < 0.001 and ****P < 0.0001 as compared to untreated cells from at least four independent experiments.
  5. Immunoblot analysis of protein lysates from THP1 cells WT or TFEB‐S401A knock‐ins (clones I11 and M17) incubated with 100 μM NaAsO2 for 1 h.
  6. Immunoblot analysis of protein lysates from PMA‐differentiated THP1 cells WT or TFEB‐S401A knock‐ins (clones I11 and M17) incubated with 1 μg/ml LPS for the indicated times. Immunoblots are representative of at least three independent experiments.

Data information: n ≥ 3 biological replicates (each dot represents a biological replicate).

Source data are available online for this figure.