Figure EV5. NONO construct lacking the N‐ and C‐terminal low complexity domain is more diffuse in the nucleus than wildtype NONO.

1. Fluorescence micrograph images of representative cells stained for NONO and NEAT1_2 in KELLY and HeLa cells transfected with GFP fused NONO_∆1–52 + ∆313–466 plasmids. Scale bar: 5 μm.
2. Construction of NONO_∆1–52 + ∆313–466 plasmids, indicating how the mutant lacking the N‐ and C‐terminal LCD is equivalent to a soluble structured dimer, previously solved by crystallography (PDB 3SDE).