Fig. 3.

Effect of all fractions (Fr. (a)–(f) and crude extracts) on LPS-mediated production of NO in RAW264 cells. Cells were pre-treated with various concentrations of Ganoderma fractions or Dex (positive control, 4 μg/mL) for 2 h and were co-treated with LPS (1 μg/mL) for 18 h. NO levels in the cell culture media were determined using a Griess assay. Data shown are expressed as the mean ± SD (n = 3). ###p < 0.001 when compared with control versus LPS; ***p < 0.001, **p < 0.01, *p < 0.05 as compared to the group treated with LPS alone.