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. 2023 Jan 20;19:100549. doi: 10.1016/j.mtbio.2023.100549

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© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 6 — Evaluation of the polarization ability of macrophages induced by different Exos in vitro and vivo. (a) Immunofluorescence staining of CD206 in macrophages treated with different Exos for 24 h. Scale bar = 20 μm. (b) RT-qPCR detection of M2 (CD163、Arg-1、CD206) marker gene expression in macrophages cultured with different Exos for 24 h (n = 3). (∗∗P < 0.01, ∗∗∗P < 0.005). (c) Quantitative analysis of the Im-munofluorescence staining (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.005, ∗∗∗∗P < 0.001). N = 3. (d) Immunofluorescence staining of CD68⁺/CD86+/CD206⁺ macrophages in synovium at 14 days after surgery. Scale bar = 20 μm. (e) Gene expression of CD206 and CD86 in synovium macrophages at 14 days (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.005) (n = 3). (f) Quantitative analysis of Immunofluorescence staining of CD86⁺/CD206⁺ macrophages in synovium (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.005, ∗∗∗∗P < 0.001). N = 3.