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. 2023 Jan 24;60:102615. doi: 10.1016/j.redox.2023.102615

Fig. 2.

Fig. 2

SIRT1 expression decreases in endothelial cells of the spinal cord after SCI. (A) Western blot analysis of SIRT1 expression in the spinal cord in sham-operated mice, and at 8 h, 1 d, 3 d, 7 d and 14 d after SCI in mice (n = 6 animals per group). (B) Quantification of relative levels of SIRT1 protein (n = 6 animals per group). (C) qRT-PCR analysis of the mRNA level of SIRT1 in the spinal cord in sham-operated mice, and at 8 h, 1 d, 3 d, 7 d and 14 d after SCI in mice (n = 6 animals per group). (D) Double immunostaining images of spinal cord sections, showing that SIRT1 is expressed mainly in endothelial cells (CD31+, white arrows) and neurons (NeuN+, white arrows), but is not expressed in astrocytes (GFAP+), oligodendrocytes (Olig2+) and microglia (Iba1+). (E) Representative immunofluorescence images of SIRT1 (red) and CD31+ (green), showing lower SIRT1 expression in endothelial cells of the spinal cord in mice at 3 d after SCI than in sham-operated mice (n = 6 animals per group). (F) Quantification of SIRT1 expression (n = 6 animals per group). (G) qRT-PCR analysis of the mRNA level of SIRT1 in isolated endothelial cells of the spinal cord, showing lower SIRT1 expression in endothelial cells of the spinal cord in mice at 3 d after SCI than in sham-operated mice (n = 3 independent experiments). *P < 0.05; ns, not significant; compared with the sham-operated group. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)