Table 1.
Statistical parameters for all FGFR3 variants tested without ligand
| Variant | Variant (CDS) | Cells | CmScarlet mean ± SE | p | CmGFP mean ± SE |
|---|---|---|---|---|---|
| K508M | c.1523A>T | 56 | 0.05 ± 0.01 | 1.27E-13 | 0.82 ± 0.01 |
| Positive CTR | None | 36 | 0.71 ± 0.01 | 6.61E-33 | 0.93 ± 0 |
| WT | None | 115 | 0.24 ± 0.02 | Not applicable | 0.85 ± 0.01 |
| G380R | c.1138G>A | 51 | 0.42 ± 0.02 | 5.86E-09 | 0.90 ± 0.01 |
| Y373C | c.1118A>G | 49 | 0.43 ± 0.03 | 3.92E-09 | 0.89 ± 0.01 |
| K650Q | c.1948A>C | 56 | 0.50 ± 0.02 | 3.20E-16 | 0.89 ± 0.01 |
| K650E | c.1948A>G | 54 | 0.35 ± 0.02 | 2.62E-04 | 0.87 ± 0.01 |
The second column indicates the position of missense mutation in the coding sequence of FGFR3 (CDS) introduced in the expression plasmid. The p value represents the pairwise comparison of the respective CmScarlet to the nonactivated WT. For the complete dataset also with the addition of the ligands fgf1 and fgf2, see Table S2.