Figure 1.

Proteomic studies suggest an intranuclear role of GIV/Girdin in DNA damage repair response

(A) Schematic outlining key steps in BioID studies to identify the GIV interactome.

(B) Immunoblots confirm biotinylation in HEK whole cell lysates (left) and expression of the BirA-tagged full-length GIV construct as a protein of expected size (right).

(C and D) Bar plots show GO analyses [cellular component (C) and molecular function (D)] for bioID-identified GIV interactome. Red bars in C indicate putative compartments where GIV binds BRCA1. Blue and red bars in D indicate a total number of interacting proteins and % representation, respectively. Red arrow in D indicates the molecular function category where BRCA1 was identified.

(E and F) DNA-binding proteins (listed in E) that were identified in GIV’s interactome were analyzed by Reactome.org and visualized as hierarchical reacfoam (in F). Inset in the top right corner is magnified to highlight the overrepresentation of DNA repair pathways.