| Instrument | |
| Grating | 1,800 grooves/mm |
| Wavelength of excitation | 532 nm |
| Object of focusing | 50× (for SERS); 10× (for mapping) |
| Detector | CCD detector |
| Spectral acquisition parameter | |
| Acquisition time | 10 s (for SERS); 3 s (for mapping) |
| Time of exposure | 10 s |
| Excitation power | ∼25 mW |
| Beam spot | ∼1.5 μm |
| Sample size | 5 mm2 |
| Detection wavelength range | 400–1,800 cm-1 |
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