Fig. 6.
Characterization and quantification of astrocyte-derived exosomes. (A) Nanoparticle tracking analysis of ultrasound-stimulated astrocytes and astrocyte-derived exosomes showing the number and size distribution and (B) Total concentrations. Total protein level of exosomes determined by SDS-PAGE (C) and BCA (D). Western blotting (E) and densitometric quantification (F) of biomarker proteins of exosomes from both ultrasound-stimulated astrocytes and astrocytes without any treatment. (G) Representative TEM of exosomes. Scale bars: 100 nm ∗ denotes significant difference compared with controls. Data are presented as means ± SD (n = 3). ∗P < 0.05; ∗∗P < 0.01. The image was reproduced from the article published in Theranostics Journal by Deng, Wang and Meng and Co-workers in the year 2021 [428]. Permission was obtained from the publisher for using the illustration.