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. 2023 Feb 1;18:563–578. doi: 10.2147/IJN.S392782

Table 1.

Quantitative Real-Time PCR Primers

Gene Name Sequence
GAPDH forward 5 ′ -CCTCGTCCCGTAGACAAAATG −3 ′
GAPDH reverse 5 ′ -TGAGGTCAATGAAGGGGTCGT-3 ′
ACTA2 forward 5 ′ -GTACCACCATGTACCCAGGC-3 ′
ACTA2 reverse 5 ′ -GAAGGTAGACAGCGAAGCCA-3 ′
COL1A1 forward 5 ′ -GAGAGGTGAACAAGGTCCCG-3 ′
COL1A1 reverse 5 ′ -AAACCTCTCTCGCCTCTTGC-3 ′
TIMP1 forward 5 ′ -GCAACTCGGACCTGGTCATAAG-3 ′
TIMP1 reverse 5 ′ -TCCCACAGCCTTGAATCCTTT-3 ′
MMP13 forward 5 ′ -CTTCTTCTTGTTGAGCTGGACTC-3 ′
MMP13 reverse 5 ′ -CTGTGGAGGTCACTGTAGACT-3 ′
VEGF forward 5 ′ -GAGCGTTCACTGTGAGCCTTGT-3 ′
VEGF reverse 5 ′ -TTAACTCAAGCTGCCTCGCCT-3 ′
TGF-β1 forward 5 ′ -TAATGGTGGACCGCAACAAC-3 ′
TGF-β1 reverse 5 ′ -CCACATGTTGCTCCACACTTGAT-3 ′
SMAD3 forward 5 ′ -GGAATGCAGCCGTGGAACTT-3 ′
SMAD3 reverse 5 ′ -TTGCAGCCTGGTGGGATCTT-3 ′
SMAD7 forward 5 ′ -GGCCGGATCTCAGGCATTC-3 ′
SMAD7 reverse 5 ′ -TTGGGTATCTGGAGTAAGGAGG-3 ′