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[Preprint]. 2023 Jan 23:2023.01.23.525149. [Version 1] doi: 10.1101/2023.01.23.525149

Fig. 6. Cryptic peptides are present in human CSF.

Fig. 6.

(A) Rank plot of proteins detected in CSF from patients with ALS. 47 cryptic exon genes that were predicted in TDP-43 KD iPSC neurons are shown in red. PRM-validated cryptic peptides expressing genes (from iNeuron studies) are annotated with blue text. (B) Venn Diagram of three CSF proteomics datasets versus cryptic exons predicted in TDP-43 KD iPSC neurons. (C) Representative spectral plots of three heavy (standard) and light (endogenous) cryptic peptides detected in ALS patient CSF. (D) MS/MS spectrum of a cryptic peptide in HDGFL2 that corresponds to the reference peptide (top) and endogenous peptide (bottom) detected in ALS patient CSF. (E) Heatmap of AUC intensities of 18 cryptic peptides from 13 different proteins in CSF from 15 ALS patients.