Fig. 3. Exacerbated diabetic mechanical allodynia in adult mice lacking skin keratinocyte-derived SIRT1.

(A) Assessment of static allodynia in keratinocyte-specific SIRT1 KO mice and two control groups: Control 1: SIRT1^flox/flox and Control 2: K5-CreER^T2;SIRT1^flox/flox receiving no tamoxifen but showing leaky Cre expression (Supplementary Fig. 4). Static allodynia was assessed by the von Frey assay. Note that only mice with normal (1 or 1.4 g) or decreased pain thresholds (< 1 g) were charted. (B) Assessment of dynamic allodynia in SIRT1 KO and control mice. Dynamic allodynia was assessed by the dynamic brush assay. (C) Mice with increased pain thresholds (≥ 2g) are charted with the rest of the mice. (D) Progression of static and (E) dynamic allodynia in SIRT1 KO and control mice. Error bars indicate SEM. Statistics were performed by two-way ANOVA: **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns = non-significant.