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[Preprint]. 2024 Mar 2:2023.01.24.523981. Originally published 2023 Jan 25. [Version 2] doi: 10.1101/2023.01.24.523981

Fig. 5. Reduced numbers and abnormal morphology of Meissner corpuscles in foot skin from mice lacking skin keratinocyte-derived SIRT1.

Fig. 5.

(A) IF images of Meissner corpuscles and IHC images of free nerve endings in foot skin from keratinocyte-specific SIRT1 KO, Control 1 and Control 2 mice. Scale bars represent 50 μm. (B) IF images of Meissner corpuscles double stained with S100 and NFH. (C) Meissner corpuscle, (D) free nerve ending (IENFD) and (E) epidermal Aβ fiber densities in foot skin from SIRT1 KO and control mice. (F-H) IF images showing aberrant Meissner corpuscles with the tip appearing as a long horizontal bar in epidermis (filled arrowheads). Note in (H) that the aberrant tip did not contain the innervating NFH+ large axons. Scale bars represent 50 μm. Scale bars represent 100 μm for b, e-g. (I) Electron micrographs demonstrating a relatively normal Meissner corpuscle (left) containing nerve endings (arrowheads) ensheathed by glial cell lamellae and an aberrant Meissner corpuscle (right) with elongated glial cell lamellae but no nerve endings. K = keratinocytes. Scale bar represents 2 μm. Error bars indicate SEM. Statistics were performed by one-way ANOVA: * p < 0.05, ns = non-significant.