Extended Data Figure 3. Differential gene expression induced by single-agent or combination treatments in a KRAS^G12C–driven non-small cell lung cancer (NSCLC) or KRAS^G12C–driven colorectal cancer (CRC) xenograft model.

All treatments were administered over 7 consecutive days, with samples collected after the last dose.

a, Biomarker analyses of cell proliferation biomarker, Ki-67 (left), and cell growth biomarker, phospho-ERK (p-ERK) (right), in NCI-H2122 xenograft models treated with vehicle (con), BI-3406 (50 mg/kg, bid, p.o.), adagrasib (100 mg/kg, qd, p.o.), TNO155 (10 mg/kg, bid, p.o.), adagrasib (100 mg/kg) plus BI-3406 (50 mg/kg), adagrasib (100 mg/kg) plus TNO155 (10 mg/kg) at 4 or 48 hours post-last dose. Control animals were treated with vehicle. N=4–5/group. Boxplots show low and upper quartiles and median line is indicates. Whiskers: 1.5 x interquartile range. Data analyzed by one-sided Mann-Whitney-Wilcoxon U-tests adjusted for multiple comparisons according to the Bonferroni-Holm Method within each subtopic. b, Biomarker analyses of cell proliferation biomarker, Ki-67 (left), and cell growth biomarker, phospho-ERK (p-ERK) (right), in SW837 xenograft models treated with vehicle (con), BI-3406 (50 mg/kg, bid, p.o.), adagrasib (100 mg/kg, qd, p.o.), TNO155 (10 mg/kg, bid, p.o.), adagrasib (100 mg/kg) plus BI-3406 (50 mg/kg), adagrasib (100 mg/kg) plus TNO155 (10 mg/kg) at 4 or 48 hours post-last dose. Control animals were treated with vehicle. N=4–5/group. Boxplots show low and upper quartiles and median line is indicates. Whiskers: 1.5 x interquartile range. Data analyzed by one-sided Mann-Whitney-Wilcoxon U-tests adjusted for multiple comparisons according to the Bonferroni-Holm Method within each subtopic.