Figure 5: TNSST neurons display increased hormonal pathway co–correlations with white adipose tissue in individuals with inferred high estrogen signaling.
(A) Schematic overview of co-correlation analysis. High expressing TNSST genes from mouse Flow-Seq experiments were co-correlated across various peripheral metabolic tissues across high and low estradiol groups identified in the GTEx database. Created with BioRender.com. (B) Inferred estradiol levels affected co-correlation pathways relevant to all secreted proteins. High estradiol individuals showed increased co-correlations in adipose tissue whereas those with lower estradiol showed increases in skeletal muscle and stomach communication. (C) Similar trends in adipose and stomach co-correlations across estradiol groupings were seen for ligand pathways. (D) Co-correlations across tissues showed differential impact on genes associated with feeding pathways. Individuals with higher estradiol showed increased co-correlations within these pathways with adipose tissue and decreased co-correlations with stomach as compared to those with lower estradiol levels.