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[Preprint]. 2023 Jul 20:2023.01.26.525697. Originally published 2023 Jan 27. [Version 2] doi: 10.1101/2023.01.26.525697

Figure 4. Analyses of lipids in crude homogenates LacQ140 caudate putamen by mass spectrometry.

Figure 4.

(a) Total lipid intensity detected at 6 months normalized to WT; no significant difference between groups (one-way ANOVA: F(3, 20) = 0.4604, P=0.7130, n.s., n=6). (b) Total lipid intensity detected at 9 months normalized to WT; LacQ140 mice have decreased total lipid intensity which is reversed in LacQ140_A mice (one-way ANOVA & Tukey’s multiple comparison test: F(3, 20) = 5.474, **P=0.0065, n=6). (c) Total lipid intensity detected at 12 months normalized to WT; no significant difference between groups (one-way ANOVA: F (4, 25) = 0.7504, P=0.5671, n.s., n=6). (d) Heatmap depicts the lipid subclass composition for WT, LacQ140, and treatment groups at 9 months. Hierarchical clustering was performed across lipid subclasses (rows) and columns (animals) using the one minus Pearson correlation distance metric. ANOVA p-value column indicates lipid subclasses significantly changed between LacQ140 and WT mice in green (p<0.05, One-way ANOVA with Tukey’s multiple comparison test, n=6). Lipid subclasses with adjusted p-values (q) < 0.05 LacQ140 vs WT are indicated in purple (q<0.05, two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli, N=24 lipid subclasses, n=6 mice). Source data and full statistical details can be found in Additional Files 1, 4, & 5.