Table 1.
Overview of lipid subclass changes at 6, 9, and 12 months
| 6-month-old mice | 9-month-old mice | 12-month-old mice | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
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| Category | Subclass | Species detected |
ANOVA p-val |
Adj p-val (q), N =36 |
Species detected |
ANOVA p-val |
Adj p-val (q), N =24 |
Species detected |
ANOVA p-val |
Adj p-val (q), N =29 |
|
| ||||||||||
| Glycerophos-pholipids | PC | 134 | 0.7415 | 1 | 107 | 0.2928 | 0.3267 | 160 | 0.4243 | 0.4627 |
| PE | 152 | 0.1975 | 1 | 154 | 0.0284* | 0.0563 | 119 | 0.5653 | 0.5372 | |
| PS | 62 | 0.1171 | 1 | 58 | 0.0014 | 0.0125 | 25 | 0.46 | 0.4627 | |
| PI | 19 | 0.0189 | 0.7144 | 17 | 0.0054 | 0.0168 | 34 | 0.0086* | 0.0338* | |
| MePC | 17 | 0.9859 | 1 | 7 | 0.4901 | 0.486 | 15 | 0.7552 | 0.6329 | |
| PA | 13 | 0.9596 | 1 | 6 | 0.7008 | 0.5957 | 9 | 0.0025* | 0.0184* | |
| BisMePA | 4 | 0.1589 | 1 | 1 | 0.0041 | 0.0168 | 6 | 0.2812 | 0.3647 | |
| dMePE | 9 | 0.195 | 1 | 2 | 0.2041 | 0.269 | 0 | N/A | N/A | |
| PG | 19 | 0.6255 | 1 | 4 | 0.4685 | 0.486 | 4 | 0.859 | 0.6765 | |
| BisMePE | 1 | 0.8072 | 1 | 0 | N/A | N/A | 0 | N/A | N/A | |
| BisMePS | 0 | N/A | N/A | 0 | N/A | N/A | 2 | 0.0001* | 0.0022* | |
| CL | 22 | 0.5812 | 1 | 0 | N/A | N/A | 0 | N/A | N/A | |
| PEt | 7 | 0.3261 | 1 | 2 | 0.9792 | 0.7283 | 0 | N/A | N/A | |
| PMe | 2 | 0.8695 | 1 | 0 | N/A | N/A | 0 | N/A | N/A | |
| PIP2 | 2 | 0.1393 | 1 | 0 | N/A | N/A | 3 | 0.0161* | 0.0394* | |
| PIP | 1 | 0.7034 | 1 | 0 | N/A | N/A | 0 | N/A | N/A | |
| LPC | 30 | 0.7943 | 1 | 10 | 0.6835 | 0.5957 | 7 | 0.2805 | 0.3647 | |
| LPE | 23 | 0.9947 | 1 | 16 | 0.6396 | 0.5957 | 4 | 0.0127* | 0.035* | |
| LPS | 7 | 0.9061 | 1 | 5 | 0.211 | 0.269 | 2 | 0.0817 | 0.1287 | |
| LPI | 7 | 0.7746 | 1 | 1 | 0.1715 | 0.2551 | 0 | N/A | N/A | |
| LPG | 4 | 0.8888 | 1 | 0 | N/A | N/A | 0 | N/A | N/A | |
| LdMePE | 1 | 0.4552 | 1 | 0 | N/A | N/A | 0 | N/A | N/A | |
| BiotinylPE | 0 | N/A | N/A | 1 | 0.8078 | 0.6554 | 1 | 0.0225* | 0.0451* | |
|
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| Glycerolipids | TG | 107 | 0.5998 | 1 | 44 | 0.0057* | 0.0168* | 72 | 0.0002* | 0.0022* |
| MGDG | 3 | 0.8693 | 1 | 2 | 0.0005 | 0.0089 | 5 | 0.0214* | 0.0451* | |
| MGMG | 6 | 0.7537 | 1 | 0 | N/A | N/A | 6 | 0.0497* | 0.0887 | |
| DG | 13 | 0.1381 | 1 | 70 | 0.1174 | 0.1905 | 59 | 0.6614 | 0.5834 | |
| SQMG | 2 | 0.5986 | 1 | 0 | N/A | N/A | 0 | N/A | N/A | |
| SQDG | 1 | 0.9316 | 1 | 0 | N/A | N/A | 0 | N/A | N/A | |
|
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| Sphingolipids | Hex1Cer | 0 | N/A | N/A | 85 | 0.0243* | 0.0542 | 104 | 0.4617 | 0.4627 |
| CerG1 | 50 | 0.5466 | 1 | 0 | N/A | N/A | 0 | N/A | N/A | |
| SM | 47 | 0.6398 | 1 | 23 | 0.0066 | 0.0168 | 36 | 0.3243 | 0.3973 | |
| Cer | 14 | 0.9804 | 1 | 6 | 0.0048 | 0.0168 | 21 | 0.4374 | 0.4627 | |
| CerP | 0 | N/A | N/A | 0 | N/A | N/A | 17 | 0.0035* | 0.0193* | |
| ST | 10 | 0.3977 | 1 | 9 | 0.2283 | 0.2717 | 14 | 0.2176 | 0.3199 | |
| So | 3 | 0.4465 | 1 | 0 | N/A | N/A | 0 | N/A | N/A | |
| CerPE | 0 | N/A | N/A | 1 | 0.0766 | 0.1367 | 0 | N/A | N/A | |
| phSM | 4 | 0.3619 | 1 | 0 | N/A | N/A | 0 | N/A | N/A | |
| CerG2GNAc1 | 1 | 0.5784 | 1 | 0 | N/A | N/A | 0 | N/A | N/A | |
| SPH | 0 | N/A | N/A | 0 | N/A | N/A | 1 | 0.775 | 0.6329 | |
| Hex2Cer | 0 | N/A | N/A | 0 | N/A | N/A | 1 | 0.0112 | 0.035 | |
|
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| Sterol Lipids | ChE | 1 | 0.5341 | 1 | 1 | 0.918 | 0.7124 | 2 | 0.9151 | 0.6958 |
| ZyE | 0 | N/A | N/A | 0 | N/A | N/A | 3 | 0.0092* | 0.0338* | |
|
| ||||||||||
| Fatty Acyls | FA | 2 | 0.5893 | 1 | 0 | N/A | N/A | 0 | N/A | N/A |
| AcCa | 0 | N/A | N/A | 0 | N/A | N/A | 2 | 0.5847 | 0.5372 | |
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| Prenol Lipids | Co | 0 | N/A | N/A | 0 | N/A | N/A | 1 | 0.0523 | 0.0887 |
|
| ||||||||||
| Total | 800 | 632 | 735 | |||||||
Bold text indicates lipid subclasses significantly different between WT and LacQ140 mice (p<0.05 or q<0.05).
*
indicates lipid subclasses significantly different among other groups (p<0.05 or q<0.05).
One way ANOVA was conducted followed by Tukey’s multiple comparisons test, two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli, q<0.05.