Fig 5. ZfpA overexpression decreases voriconazole efficacy in vitro and during infection.

(A) Susceptibility of WT CEA10, ΔzfpA, and OE::zfpA to 0.1 and 0.25 μg/mL voriconazole (VOR). 10⁴ spores were point-inoculated on solid GMM with voriconazole or DMSO. Images are representative of colony growth 4 days post inoculation. Bars represent mean±s.d. of relative colony diameter of 4 plates per condition. p values calculated by ANOVA with Tukey’s multiple comparisons. ***p<0.001, ****p<0.0001. (B) Survival analysis of infected Rac2D57N larvae (neutrophil-deficient) bathed in 0.1 μg/mL voriconazole or 0.001% DMSO. Average spore dose injected: WT CEA10 = 39 , ΔzfpA = 35, OE::zfpA = 37. Results represent pooled data from 3 independent experiments. n = 44-66 larvae per condition. p values and hazard ratios calculated by Cox proportional hazard regression analysis.