Table 3.
Primers used in this study
| Name | 5’ -> 3’ | Use |
|---|---|---|
| zfpA5’F | TGACCATGATCTCCACTTCCCC | zfpA deletion |
| zfpA5’R | CGATATCAAGCTATCGATACCTCGACTCGC AGACGTCCTAAGCTCGATAGTCGACTG |
zfpA deletion |
| parapyrGF | GAGTCGAGGTATCGATAGCTTG | zfpA deletion |
| parapyrGR | ATTCGACAATCGGAGAGGCTGC | zfpA deletion |
| zfpA3’F: | GTCGCTGCAGCCTCTCCGATTGTCGAATCG ACGATGAACCTGAGGAAGATGACGACG |
zfpA deletion |
| zfpA3’R: | GATACTTTTCAGCTGCAGCCGC | zfpA deletion |
| zfpAkoconfF: | CACAGCGCATAAAACCATCGCC | Confirmation of zfpA deletion |
| zfpAkoconfR: | TAGGGCCTATCCTTAGGGTACC | Confirmation of zfpA deletion |
| zfpAOE5’F | zfp5’F recycle | zfpA overexpression |
| zfpOE5’R | CCAATTCGCCCTATAGTGAGTCGTATTACG GCAGACGTCCTAAGCTCGATAGTCGACTG |
zfpA overexpression |
| OEPyGF: | CGTAATACGACTCACTATAGGGC | zfpA overexpression |
| OEPyGR: | GGTGATGTCTGCTCAAGCGGG | zfpA overexpression |
| zfpOE3’F: | CAGCTACCCCGCTTGAGCAGACATCACCAT GCAGAGCCCAGGAGAACATTCCGAC |
zfpA overexpression |
| zfpOE3’R: | GTATTCGCACGTAACGATGGGG | zfpA overexpression |
| zfpAOEconfF | ATTCATCTTCCCATCCAAGAACC | Confirmation of zfpA overexpression |
| zfpOEconfR | TGTTTGCTCAACGCCATGCACG | Confirmation of zfpA overexpression |
| afumipyrGF | CTACCTCGAGAATATGCCTCAAAC | pyrG-complementation |
| afumipyrGR | GGCGACTTATTCTGTCTGAGAG | pyrG-complementation |