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. 2023 Feb 5;24:42. doi: 10.1186/s12931-023-02328-2

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — PIEZO1 and PIEZO2 mediate the effects of manipulating intraluminal chloride. a–c Upper panel represents the main cumulative effect of intraluminal injection of distinct chloride concentrations ([Cl⁻]) 5.8, 143, or 715 mM Cl⁻ and the medium supplementation with GsMTx4 at day0 (D0) and day2 (D2). a Represents lung explants at D0 and D4 for the different [Cl⁻]. b, c Morphometric analysis of b peripheral airway buds and c epithelial perimeter. d–f Lower panel shows the additional effect of PIEZO1/2 inhibition after intraluminal injection of Cl⁻ channels inhibitors: cystic fibrosis transmembrane conductance regulator inhibitor172 (CFTRinh) to CFTR; and calcium-dependent Cl⁻ channel inhibitor A01 (CaCCinh) to CaCCs. d represents the fetal lung explants at D0 and D4 for the distinct Cl⁻ channels inhibitors. e, f Morphometric analysis of e peripheral airway buds and f epithelial perimeter. 143 mM Cl⁻ and standard solution (SS) identified the control condition for [Cl⁻] and Cl⁻ channels inhibitors, respectively. White and dotted rectangles represent the medium supplementation with and without GsMTx4, respectively. n ≥ 4 were used per antibody/condition. Results are expressed as the ratio of D4 and D0 (D4/D0) and presented as mean ± SD. Symbols indicate the main effects and non-redundant interactions of the two-way ANOVA. p < ^α0.0001, ^β0.001, ^γ0.01, ^µ0.05