Fig. 5.

Decrease of PIEZO1 and PIEZO2 expression removes the molecular dynamics triggered by intraluminal chloride concentration. a–e Upper panel represents the main cumulative effect of intraluminal chloride concentration ([Cl⁻]) and medium supplementation with (dotted rectangles) and without (white rectangles) GsMTx4. a Examples of representative blots are shown. b–e Protein expression levels for b PIEZO1, c PIEZO2, d bombesin, and e ghrelin are indicated. f–j Lower panel shows the additional effect of GsMTx4 after intraluminal injection of Cl⁻ channels inhibitors: cystic fibrosis transmembrane conductance regulator inhibitor172 (CFTRinh) to CFTR; and calcium-dependent Cl⁻ channel inhibitor A01 (CaCCinh) to CaCCs. f Examples of representative blots are shown. g–j Relative expression levels of g PIEZO1, h PIEZO2, i bombesin, and j ghrelin. 143 mM Cl⁻ and standard solution (SS) represent the control condition for [Cl⁻] and Cl⁻ channels inhibitors, respectively. n ≥ 4 were used per antibody/condition. Results are presented as mean ± SD. Symbols indicate the main effects and non-redundant interactions of the two-way ANOVA. p < ^α0.0001