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. Author manuscript; available in PMC: 2023 Feb 6.
Published in final edited form as: Lab Invest. 2008 Oct 6;88(12):1264–1277. doi: 10.1038/labinvest.2008.98

Figure 2.

Figure 2

Endogenous expression levels of osteogenic regulators and other osteogenic markers in human osteosarcoma cells. Four OS lines, 11 primary OS lines, human osteoblast line hFOB, and primary human marrow mesenchymal line hMSC were cultured in 1% FCS for 36 h, and were collected for total RNA isolation. The total RNA was subjected to reverse transcription and subsequently quantitative real-time PCR (qPCR) analysis. Gene-specific primers (listed in Supplementary Table 1) were used to determine the expression levels of osteogenic regulators Runx2 (a) and Osterix (OSX) (b), early osteogenic marker CTGF/CCN2 (c), and late osteogenic marker osteopontin (OPN) (d). All qPCR samples were normalized with the expression level of GAPDH. Each condition was done in triplicate. Dotted lines indicate the expression levels of the indicated genes in hFOB, a mature osteoblast line. See ‘Materials and Methods’.