Figure 11.

Phylogeny of phage vB_Kpn_ZCKp20p. (A) VICTOR genome-based phylogenetic tree conducted by nucleotides pairwise comparison of phage vB_Kpn_ZCKp20p, other Caudoviricetes phages, and closely related phages according to genome BLASTn top hits. The phages were clustered into 6 families, 10 genera, and they were clustered into 94 species. The GC content of the phage genomes is represented in the hue of blue color, and the genome size is presented in horizontal black lines on the right side. (B) VIRIDIC heatmap for the intergenomic similarity between phage vB_Kpn_ZCKp20p and closely related phages according to genome BLASTn top hits. Three factors of the alignment capacity were considered to calculate the relatedness between the phages: intergenomic similarities (hue of green to blue), the percentage of aligned genome sequence in a pair (shade of pink) and their length ratio (shade of black). (C) PhageCloud analysis of phage vB_Kpn_ZCKp20p genomic relationship with the closest reference phage genomes on NCBI-GenBank. Intergenomic distances were calculated by dashing based on a threshold of 0.25.